Galvani, as he himself describes, proceeded with immense enthusiasm to investigate systematically what accident had thus put into his hands.² He wanted first to see whether changes occurring naturally in the electrical condition of the atmosphere would call forth the same reaction in his specimens. For this purpose he fastened one end of an iron wire to a point high up outside his house; the lower end he connected with the nervous substance of a limb from one of his specimens, and to the foot of this he attached a second wire whose other end he submerged in a well. The specimen itself was either enclosed in a glass flask in order to insulate it, or simply left lying on a table near the well. And all this he did whenever a thunderstorm was threatening. As he himself reported: 'All took place as expected. Whenever the lightning flashed, all the muscles simultaneously came into repeated and violent twitchings, so that the movements of the muscles, like the flash of the lightning, always preceded the thunder, and thus, as it were, heralded its coming.' We can have some idea of what went on in Galvani's mind during these experiments if we picture vividly to ourselves the animal limbs twitching about every time the lightning flashed, as if a revitalizing force of will had suddenly taken possession of them.